Recombinant Human IL-7
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BackgroundInterleukin 7 (IL-7), also known as Lymphopoietin 1, is a hematopoietic growth factor which affects primarily early B and T cells (1). It stimulates the differentiation of multipotent hematopoietic stem cells into lymphoid progenitor cells. IL-7 is important for proliferation during certain stages of B cell maturation, T and NK cell survival, development and homeostasis. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2 (2). In myeloid lineage cells, IL-7 up-regulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes and macrophages. IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections (3). Human and murine IL-7 is cross-species reactive. Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01EU/µg as determined by the LAL method Biological Activity The biological activity of Human IL-7 was Measured as its T cell growth factor activity in a cell proliferation assay using PHA activated human peripheral blood lymphocytes (Yokota, T. et al., 1986, Proc. Nat. Acad. Sci. USA 88:5894). The expected ED<sub>50</sub> for this effect is typically 0.2 - 0.5 ng/ml. Protein Accession No. Amino Acid Sequence mdcdie gkdgkqyesv lmvsidqlld smkeigsncl nnefnffkrh icdankegmf lfraarklrq flkmnstgdf dlhllkvseg ttillnctgq vkgrkpaalg eaqptkslee nkslkeqkkl ndlcflkrll qeiktcwnki lmgtkeh N-terminal Sequence Analysis Met State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human IL-7 is Mr 17 kDa. Predicted Molecular Mass 17 Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives present. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Namen, AE. et al. (1990) Int. J. Cell Cloning 1:168 2. Reed, SG. et al. (1989) J. Exp. Med. 169:707 3. Prakash, S. et al. (2008) Appl. of Biochem. Biotechnol. 151:93 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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