Recombinant Human IL-7

Recombinant Human IL-7

Product No.: I-189

[product_table name="All Top" skus="I-189"]

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Alternate Names
Interleukin-7 Lymphopoietin 1, LP-1, Pre-B Cell Factor
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human

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Background

Interleukin 7 (IL-7), also known as Lymphopoietin 1, is a hematopoietic growth factor which affects primarily early B and T cells (1). It stimulates the differentiation of multipotent hematopoietic stem cells into lymphoid progenitor cells. IL-7 is important for proliferation during certain stages of B cell maturation, T and NK cell survival, development and homeostasis. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2 (2). In myeloid lineage cells, IL-7 up-regulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes and macrophages. IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections (3). Human and murine IL-7 is cross-species reactive.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-7 was Measured as its T cell growth factor activity in a cell proliferation assay using PHA activated human peripheral blood lymphocytes (Yokota, T. et al., 1986, Proc. Nat. Acad. Sci. USA 88:5894). The expected ED<sub>50</sub> for this effect is typically 0.2 - 0.5 ng/ml.
Protein Accession No.
Amino Acid Sequence
mdcdie gkdgkqyesv lmvsidqlld smkeigsncl nnefnffkrh icdankegmf lfraarklrq flkmnstgdf dlhllkvseg ttillnctgq vkgrkpaalg eaqptkslee nkslkeqkkl ndlcflkrll qeiktcwnki lmgtkeh
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-7 is Mr 17 kDa.
Predicted Molecular Mass
17
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives present.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Namen, AE. et al. (1990) Int. J. Cell Cloning 1:168
2. Reed, SG. et al. (1989) J. Exp. Med. 169:707
3. Prakash, S. et al. (2008) Appl. of Biochem. Biotechnol. 151:93

Certificate of Analysis

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.