Recombinant Human IL-9

Interleukin-9 (IL-9), also known as P40, is an immunoregulatory cytokine produced by IL-2 activated Th2 lymphocytes (1). This cytokine stimulates cell proliferation and prevents apoptosis. IL-9 functions through the IL-9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes (2). It enhances the proliferation of T lymphocytes, mast cells, erthroid precursor cells and megakaryoblastic leukemia cell lines. Th2 cells have historically been thought to be the source of IL-9. However, recent data suggest the existence of a Th2-related cell type that is characterized by the secretion of IL-9 and IL-10 (3). These so-called Th9 cells can differentiate from Th2 cells in the presence of TGF-β, or they can differentiate from a naïve CD4 cell with a combination of IL-4 and TGF-β. IL-9 plays a role in the complex pathogenesis of bronchial hyperresponsiveness as a risk factor for asthma (4). Over-expression of IL-9 has been implicated in the pathogenesis of anaplastic lymphoma and Hodgkin's disease (5).

The biological activity of Human IL-9 was determined by its ability to stimulate cell proliferation in the factor-dependent human megakaryocytic leukemic cell line, M07e.⁶ The expected ED₅₀ for this effect is typically 0.03-0.3 ng/mL.

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Gln19 predicted

Lyophilized

The predicted molecular weight of Recombinant Human IL-9 is Mr 14.1 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 12-24 kDa.

14.1

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Renauld, JC. et al. (1995) J. Immunol. 154:5061
2. Yang, YC. et al. (1995) J. Biol. Chem. 270:20497
3. Veldhoen, M. et al. (2008) Nature Immunol. 9:1341
4. Levitt, RC. et al. (1997) Proc. Natl. Acad. Sci. (USA) 94:13175
5. Gruss, HJ. et al. (1992) Cancer Res. 52:1026
6. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.