Recombinant Human Leptin R

Leptin receptor, also known as LEPR, and CD295 (cluster of differentiation 295) is a single-transmembrane-domain receptor of the cytokine-receptor family.¹ Leptin receptor exists in multiple forms with a common extracellular domain and a variable length cytoplasmatic portion. Leptin receptor may act as a negative regulator of Leptin activity and it may maintain a pool of available bioactive Leptin by binding and delaying its clearance from circulation. Leptin receptor is considered a physiological marker of primate fetal lung maturity.²

The biological activity of Human Leptin R was determined by its ability to neutralize the activity of recombinant Human Leptin on leptin-dependent human OB-R transfected murine BaF3 cells. The expected ED₅₀ is typically 0.02 - 0.12 μg/ml in the presence of 3 ng/ml rhLeptin.

Fc Fusion Protein

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Thr20

Lyophilized

The predicted molecular weight of Recombinant Human Leptin R is Mr 121 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 155-175 kDa.

121

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Guy-Grand, B. et al.(1998) Nature 392: 398
2. Castracane, VD. et al.(2004) Reproduction 127: 87