Recombinant Human LIF Rα

Leukemia inhibitory factor receptor alpha (LIF Rα), also known as CD118, is a subunit of the receptor for leukemia inhibitory factor LIF, which is a pleiotropic cytokine affecting the differentiation, survival and proliferation of a wide variety of cells in the adult and the embryo (1). LIF action is mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: LIF Rα that binds LIF with low affinity and the gp130 subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. The gp130 subunit was first described as the signal transducing subunit of the high-affinity IL-6 receptor complex (2). Signaling for the LIF complex is achieved through activation of the JAK-STAT pathway. The LIF complex also mediates the activities of oncostatin M (OSM) (3), cardiotropin-1 and ciliary neurotrophic factor (CNTF) (4). Soluble LIF Rα has been shown to bind LIF and has LIF antagonistic activities. Defects in LIF Rα are the cause of Stueve-Wiedemann syndrome (SWS), a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias (5).

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Lyophilized

The predicted molecular weight of Recombinant Human LIF Rα is Mr 89 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 100-110 kDa.

89

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Meng, L. et al.(1995) Nature378:724–727
2. Hilton, DJ. et al.(1988) J. of Biol. Chem. 263: 9238