Recombinant Human MCP-4

Monocyte chemoattractant protein-4 (MCP-4), also know as CCL13, is a member of a distinct, structurally-related subclass of CC chemokines mainly involved in recruitment of eosinphils to inflammatory sites. MCP-4 is a major chemoattractants for eosinophils, basophils monocytes and T lymphocytes. The MCP protein family bind to specific G-protein-coupled receptors, initiating a signal cascade within the cell^1,2 MCP-4 can cause joint destruction in rheumatoid arthritis.³

The biological activity of Human MCP-4 was determined by by its ability to chemoattract 2 day cultured human monocytes (Matsushima, K. et al., 1989, J. Exp. Med. 169:1485) and by its ability to chemoattract hCCR2A transfected mouse BaF/3 cells. The expected ED₅₀ for these effects are typically 0.2 - 0.6 μg/ml and 0.5 - 2 μg/ml, respectively.

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Gln24

Lyophilized

The predicted molecular weight of Recombinant Human MCP-4 is Mr 8.6 kDa.

8.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Luster, AD. et al. (1996) J Immunol. 157: 5613
2. Schleimer, RP. et al. (1997) J Clin Invest. 99: 926
3. Momohara, S. et al. (2005) Rheumatology 10: 1093