Recombinant Human MIP-1α

Macrophage inflammatory protein 1 alpha (MIP-1α), also known as CCL3, is a member of the chemokine family (1). Its primary function is as a monocyte chemoattractant, but reports have also demonstrated chemoattractant and adhesive effects by MIP-1α on CD8 T cells, B cells and eosinophils. MIP-1α has been identified as a stem cell inhibitory factor that can block the proliferation of hematopoietic stem cells (2). The functional receptor for MIP-1α is CCR1 and CCR5 and it is has been shown to interact with CCL4 (3). Following acute lung injury in rats, MIP-1α stimulates production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx (4). MIP-1α may have a potential role in host defense as a direct antiviral agent. It has been reported to show a significant direct antiviral activity against HSV-1(5).

The biological activity of Human MIP-1α was determined by its ability to chemoattract cultured human monocytes or mouse BaF3 cells transfected with human CCR5. The expected ED₅₀ for these effects are typically 2 - 10 ng/ml and 3 - 10 ng/ml, respectively.

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Ala27

Lyophilized

The predicted molecular weight of Recombinant Human MIP-1α is Mr 7.5 kDa.

7.5

This recombinant protein solutiion is supplied as a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

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1. Menten, P. et al. (2003) Cytokine Growth Factor Rev. 13:455
2. Cook, DN. et al. (1996) J. Leuko. Biol. 59:61
3. Kaufmann, A. et al. (2001) J. Leuko. Biol. 69:248
4. Ward, PA. et al. (1995) J. Immunol. 154:4793
5. Nakayama, T. et al. (2006) Virology 350:484