Recombinant Human NT-3

Neurotrophin 3 (NT-3), also known as NGF-2 and HDNF, is a basic protein and member of the NGF family. It is structurally related to β-NGF, BDNF and NT-4 (1), is expressed by neurons of the central nervous systems and can signal through the Trk receptors (2). NT-3 is also expressed in muscles and its expression is down-regulated in denervated muscles. It rapidly potentiates the spontaneous and impulse-evoked synaptic activity of developing neuromuscular synapses in culture and thus appears to be involved in the regulation of functions of developing synapses (3). NT-3 negatively modulates NGF receptor expression and associated nociceptive phenotype in intact neurons, suggesting a beneficial role in treating aspects of neuropathic pain mediated by NGF. In addition, NT-3 is effective at suppressing thermal hyperalgesia associated with chronic constriction injury (CCI) (4). NT-3 affects development of neurons in the embryo when it is expressed in human placenta. Mice born without the ability to make NT-3 show the loss of pro-prioceptive including subsets of mechanoreceptive sensory neurons (5). The amino acid sequences of human, murine and rat NT-3 are identical.

The biological activity of Human NT-3 was determined by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD. The expected ED₅₀ for this effect is typically 10 - 30 ng/ml.

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Tyr139

Lyophilized

The predicted molecular weight of Recombinant Human NT-3 is Mr 13.7 kDa.

13.7

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Barde, YA. et al. (1990) Prog. Growth Factor Res. 2:237
2. Tessarollo, L. et al. (1993) Development 118:463
3. Maisonpierre, PC. et al. (1990) Science 247:1446
4. Valerie, MK. et al. (2005) J. Neuroscie. 25:758
5. Tessarollo, L. et al. (1994) Proc. Natl. Acad. Sci. (USA) 91:11844