Recombinant Human NT-4

Neurotrophin 4 (NT-4), also known as NT-5, is a member of the NGF family and is related to β-NGF, BDNF and NT-3.¹ It promotes the development and survival of selected peripheral and CNS neurons. Several aspects distinguish NT-4 from other members of the neurotrophin family. It is the most divergent member and, in contrast to the other neurotrophins, its expression is ubiquitous and appears to be less influenced by environmental signals. NT-4 levels are highest in the prostate, with lower levels in thymus, placenta and skeletal muscle. It signals predominantly through the TrkB receptor tyrosine kinase. Knock-out mice lacking expression of NT-4 show long-term memory defects.³ In addition, NT-4 promotes the proliferation of keratinocytes and accelerates hair follicle regression.⁴ NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration.⁵

The biological activity of Human NT-4 was determined by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD. The expected ED₅₀ for this effect is typically 5 - 15 ng/ml.

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Gly81

Lyophilized

The predicted molecular weight of Recombinant Human NT-4 is Mr 14 kDa.

14

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Persson, H. et al. (1993) Development 117:1345
2. Ibanez, CF. et al. (1996) Neurochem. Sci. 21:787
3. Xie, CW. et al. (2000) Proc. Natl. Acad. Sci. (USA) 97:8116
4. Botchkarev, VA. et al. (1999) Lab. Invest. 79:557
5. Laurenzi, MA. et al. (1988) J. Leukoc. Biol. 64:228