Recombinant Human Oncostatin M

Oncostatin M (OSM) is a pleitropic cytokine produced by activated T lymphocytes and monocytes that is structurally and functionally related to the subfamily of cytokines known as the IL-6-type cytokine family.¹ OSM either inhibits or enhances the growth of cells depending on cell type. It inhibits proliferation of skeletal muscle cells by blocking cell cycle progression from G(1) to S phase.² OSM is also a key mediator for proliferation and anti-apoptosis of hepatocytes which suggests that OSM gene therapy is useful, as preventive and curative means, for the treatment of patients with liver damage.³

The biological activity of Human OSM was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323-334). The expected ED₅₀ for this effect is typically 0.15 - 0.3 ng/ml.

aaigscskey rvllgqlqkq tdlmqdtsrl ldpyiriqgl dvpklrehcr erpgafpsee tlrglgrrgf lqtlnatlgc vlhrladleq rlpkaqdler sglniedlek lqmarpnilg lrnniycmaq lldnsdtaep tkagrgasqp ptptpasdaf qrklegcrfl hgyhrfmhsv grvfskwges pnrsrr

Lyophilized

The predicted molecular weight of Recombinant Human OSM is Mr 22 kDa.

22

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Gómez-Lechón, MJ. et al. (1999) Life Sci. 65: 2019
2. Jo, SA. et al. (2008) Cell Signal 20: 120
3. Tsujimura, T. et al. (2007) Am J Pathol. 171: 872