Recombinant Human Plasma Selectin (P-selectin)

Plasma Selectin (P-selectin), also known CD62P and GMP-140, is a cell adhesion molecule and a member of the Selectin family. It is found in granules in endothelial cells and activated platelets. In unactivated endothelial cells, it is stored in granules Weibel-Palade bodies and α-granules in unactivated platelets (1). The P-selectin ligand, PSGL-1, plays an essential role in the initial recruitment of leukocytes to the site of injury during inflammation. During inflammation, when endothelial cells are activated by molecules such as histamine or thrombin, within minutes, P-selectin moves from an internal cell location to the endothelial cell surface. Thrombin is one trigger which can stimulate endothelial-cell release of P-selectin and recent studies suggest an additional Ca2+-independent pathway involved in the release of P-selectin (2). Acting in cooperation with L-selectin, P-selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic “rolling” of the leukocytes on the endothelium (3). P-selectin-mediated adhesion is an important factor in the development of early atherosclerotic lesions (1).

Measured by its ability to bind P-Selectin ligands on U937 cells. P-Selectin/Fc immobilized at 10 μg/ml will induce greater than 50% adhesion on U937 cells (100 μl/well at 1 x 106 cells/ml).

Fc Fusion Protein

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Trp42

Lyophilized

The predicted molecular weight of Recombinant Human P-Selectin is Mr 106 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 146-160 kDa under reducing conditions.

106

This recombinant protein was lyophilized from a 0.2 μm filtered solution in Tris-Citrate.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Wagner, DD. et al. (1997) J. Clin. Invest. 99:1037
2. Cleator, JH. et al. (2006) Blood 107:2736
3. Von Andrian, UH. et al. (1991) Proc. Natl. Acad. Sci. (USA) 88:7538