Recombinant Human PDGF-AB

Platelet-derived growth factor AB (PDGF-AB) is a heterodimeric¹ non-glycosylated polypeptide isomer of PDGF.² The biological effects of PDGF are mediated by cell surface alpha and beta PDGF receptors, which, as a result of ligand binding, undergo dimerization.³ PDGF-AB interaction with the alpha PDGF receptor induces conformational changes in the ligand or receptor that facilitates efficient recruitment of beta PDGFR by this PDGF isoform.⁴ However, PDGF-AA is not likely to be physiologically significant activators of cells unless the cells express PDGFR alpha.⁵ PDGF-AB may promote wound healing by stimulating extracellular matrix synthesis, e.g. collagen. It may also play a role in scar formation through collagen deposition.⁶

The biological activity of Human PDGF-AB was determined by its ability to stimulate proliferation of NR6R-3T3 fibroblasts (Raines, E.W. et al., 1985, Methods Enzym. 109:749). The expected ED₅₀ for this effect is typically 1.0 - 3.0 ng/mL when measured by 3H-thymidine incorporation.

P04085.1 (A chain) & P01127.1 (B chain)

(Human PDGF-A) sieeavpavc ktrtviyeip rsqvdptsan fliwppcvev krctgccnts svkcqpsrvh hrsvkvakve yvrkkpklke vqvrleehle cacattslnp dyreedtgrp resgkkrkrk rlkpt, (Human PDGF-B) slgsltiaep amiaecktrt evfeisrrli drtnanflvw ppcvevqrcs gccnnrnvqc rptqvqlrpv qvrkieivrk kpifkkatvt ledhlackce tvaaarpvt

Ser87 (A chain) & Ser82 (B chain)

Lyophilized

The predicted molecular weight of Recombinant Human PDGF-AB is 14.3 kDa (A chain) & 12.3 kDa (B chain). However, the actual molecular weight as observed by migration on SDS-PAGE is 17.5 kDa (A chain) & 12.5 kDa (B chain) under reducing conditions.

14.3 kDa (A chain) & 12.3 kDa (B chain)

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Helden, CH. et al. (1988) J Biol Chem. 263: 16493
2. Canalis, E. et al. (1994) Endocrinology 134: 2541
3. Giese, NA. et al. (1993) J Biol Chem. 268: 3625
4. Aaronson, SA. et al. (1991) J Biol Chem. 266: 20232
5. Bowen-Pope, DF. et al. (1993) J Biol Chem. 268: 4473
6. Xu, M. et al. (2000) Chinese Journal of Plastic Surgery and Burns 16: 78