Recombinant Human PDGF-BB

Platelet-derived growth factor-BB homodimers (PDGF-BB), also known as GDGF and ODGF, is a member of the PDGF family. PDGFs are disulfide-linked dimers consisting of two polypeptide chains, designated PDGF-A and PDGF-B. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. They are stored in platelet α-granules and are released upon platelet activation. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β (1). PDGFR-α is a high-affinity receptor for each of the three PDGF forms, while PDGFR-β interacts with only PDGF-BB and PDGF-AB. PDGF functions as a local autocrine and paracrine growth factor (2). PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of M-CSF production in vascular smooth muscle cells (3). It is also a potent wound-healing hormone that accelerates incisional repair (4). PDGF-BB may induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell migration and proliferation. It does that through agent-specific regulation of oxidative status and utilization of different signal transduction pathways (5). PDGF-BB is associated with excessive cell migration, proliferation and many growth-related diseases and may be a new prognostic indicator for patients with colorectal cancer (5-6).

The biological activity of Human PDGF-BB was determined by its ability to stimulate proliferation of NR6R-3T3 fibroblasts (Raines, E.W. et al., 1985, Methods Enzym. 109:749). The expected ED₅₀ for this effect is typically 1.5 - 6 ng/ml.

Q6FHE7

Ser82

Lyophilized

The predicted molecular weight of Recombinant Human PDGF-BB is Mr 12.4 kDa.

12.4

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Pfeilschifter, J. et al. (2005) Am. Soc. Nephrol. 10:1681
2. Claesson-Welsh, L. et al. (1991) J. Biol. Chem. 266:21138
3. Yamada, N. et al. (1992) J. Biol. Chem. 267:15455
4. Mustoe, TA. et al. (1991) J. Lab. Clin. Med. M 117:373
5. Bayraktutan, U. et al. (2008) J. Mol. Cell. Cardiol. 45:198
6. Mori, M. et al. (2008) Ann. Surg. Oncol. 15:2129