Recombinant Human PDGF Rβ

Platelet-derived growth factor receptor-β (PDGFRβ) is a well-characterized plasma membrane receptor with endogeneous tyrosine kinase activity, which is autophosphorylated in response to binding of dimeric platelet-derived growth factor (PDGF) ligand.¹ PDGFRβ mediates signaling important for the proliferation, migration, and survival of mesenchymal cells.² PDGFRβ is found in all cell types ³ and is activated in breast carcinoma cells and indicate a possible role of the receptor in breast cancerogenesis.⁴

The biological activity of Human PDGF Rβ was determined by its ability to inhibit the PDGF-BB induced 3H-thymidine incorporation in the NR6R-3T3 fibroblast cell-line (Raines, E.W. et al., 1985, Methods Enzymol. 109:749). The ED₅₀ for this effect is typically 1 - 3 μg/ml in the presence of 4 ng/ml rhPDGF-BB.

Fc Fusion Protein

lvvtppgp elvlnvsstf vltcsgsapv vwermsqepp qemakaqdgt fssvltltnl tgldtgeyfc thndsrglet derkrlyifv pdptvgflpn daeelfiflt eiteitipcr vtdpqlvvtl hekkgdvalp vpydhqrgfs gifedrsyic kttigdrevd sdayyvyrlq vssinvsvna vqtvvrqgen itlmcivign evvnfewtyp rkesgrlvep vtdflldmpy hirsilhips aeledsgtyt cnvtesvndh qdekainitv vesgyvrllg evgtlqfael hrsrtlqvvf eayppptvlw fkdnrtlgds sageialstr nvsetryvse ltlvrvkvae aghytmrafh edaevqlsfq lqinvpvrvl elseshpdsg eqtvrcrgrg mpqpniiwsa crdlkrcpre lpptllgnss eeesqletnv tyweeeqefe vvstlrlqhv drplsvrctl rnavgqdtqe vivvphslpf eniegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgkh hhhhh

Leu33

Lyophilized

The predicted molecular weight of Recombinant Human PDGF Rβ is Mr 84 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 150 kDa.

84

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Gilliland, DG. et al. (1996) Proc Natl Acad Sci USA 93: 14845
2. Ronnstrand, L et al. (1998) Biochim Biophys Acta 1378: F79
3. Fischer, B. et al. (1997) Acta Anatomica 159: 194
4. Valius, M. et al. (2005) Biologija 1: 61