Recombinant Human PEDF

Pigment epithelium derived factor (PEDF) is non-inhibitory serpin with neurotrophic and anti-angiogenic functions.¹ It protects immature but not mature granule cells against both natural and induced apoptosis.² PEDF plays an important role in regulation of glial function and proliferation in the central nervous system.³ PEDF also plays important roles in the survival and maintenance of spinal motor neurons in their neuroprotection against acquired insults in postnatal life.⁴ It is suggested that PEDF may be of therapeutic use, especially in retinopathies where pathological neovascularization compromises vision and leads to blindness.⁵

MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP

Lyophilized

The molecular weight of Recombinant Human PEDF is Mr 44.5 kDa.

The protein was 0.2 µm filtered and lyophilized from PBS.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Zhang SS et al. (2005) J Struct Biol. 151: 130 2. Schwartz JP et al. (1998) J Neurosci Res. 53: 7 3. Schwartz JP et al. (1997) J Neurosci Res. 49: 710 4. Kuncl RW. et al. (1999) J Neuropathol Exp Neurol. 58: 719 5. Bouck NP et al. (1999) Science 285: 245