Recombinant Human SCF

Stem cell factor (SCF), also known as KIT ligand, MGF and steel factor, is a type I transmembrane glycoprotein and hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis (1). It is produced by the Sertoli cells and is involved in the local regulation of spermatogenesis and liver regeneration (2). Non-covalent forms of SCF interact with the receptor tyrosone kinase c-Kit (CD117) to trigger receptor dimerization and signaling (3). The activation of c-Kit subsequently activates multiple signaling cascades, including the RAS/ERK, PI3-Kinase, Src kinase and JAK/STAT pathways (4). SCF, in conjunction with IL-31, may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung (5). It also mediates cell to cell adhesion, again by acting synergistically with other cytokines. A soluble form of SCF is produced by proteolytic processing of the molecule in the extracellular domain (6). Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells.

The biological activity of Human SCF was measured in a cell proliferation assay using a human factor-dependent cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED₅₀ for this effect is typically 2.5 - 10 ng/ml.

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Met

Lyophilized

The predicted molecular weight of Recombinant Human SCF is Mr 18.6 kDa.

18.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Toppari, J. et al. (1999) Endocrin 140: 1492
2. Adamson, JW. et al. (1991) PNAS 88: 7420
3. Hervé, L. et al. (2005) J of Endocrin. 186: 131
4. Ramadori, G. et al. (2009) Lab Invest. 89(5):562-74.
6. Phan, SH. et al. (2009) AM J Pathol. 174: 390