Recombinant Human SDF-1β
Recombinant Human SDF-1β
Product No.: S110
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Alternate Names Stromal Cell-Derived Factor-1β, Thymic Lymphoma Cell Stimulating Factor-Beta (TLSF-β), CXCL12, Pre-B Cell Growth-Stimulating Factor (PBSF) Product Type Recombinant Protein Expression Host E. coli Cells Species Human/Feline |
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BackgroundSDF-1 beta (stromal cell-derived factor-1 beta) is a non-glycosylated Polypeptide that is produced in E-Coli, belonging to the alpha chemokine (C-X-C) family of cytokines.1 SDF-1beta is a highly efficacious chemoattractant for resting lymphocytes and CD34(+) progenitor cells, and it efficiently blocks the CXCR-4-mediated entry into cells of T cell line tropic strains of HIV type 1 (HIV-1). SDF-1beta promotes HIV-1 replication and disease progression.2 Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human SDF-1β was determined by its ability to chemoattract mouse BaF/3 cells transfected with hCXCR-4 and
human T-lymphocytes cultured for 5 - 10 days. The expected ED<sub>50</sub> for these effects are typically 1-5 ng/ml and 10-30 ng/ml, respectively. Protein Accession No. Amino Acid Sequence kpvslsyrc pcrffeshva ranvkhlkil ntpncalqiv arlknnnrqv cidpklkwiq eylekalnkr fkm
N-terminal Sequence Analysis Lys22 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human SDF-1β is Mr 8 kDa. Predicted Molecular Mass 8 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA). Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Muller, HW. et al. (2000) Eur J Neurosci. 12: 1857 2. Nagai, Y. et al. (1998) Proceedings of National Acad Sci USA 95: 6331 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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