Recombinant Human SDF-1β

SDF-1 beta (stromal cell-derived factor-1 beta) is a non-glycosylated Polypeptide that is produced in E-Coli, belonging to the alpha chemokine (C-X-C) family of cytokines.¹ SDF-1beta is a highly efficacious chemoattractant for resting lymphocytes and CD34(+) progenitor cells, and it efficiently blocks the CXCR-4-mediated entry into cells of T cell line tropic strains of HIV type 1 (HIV-1). SDF-1beta promotes HIV-1 replication and disease progression.²

The biological activity of Human SDF-1β was determined by its ability to chemoattract mouse BaF/3 cells transfected with hCXCR-4 and human T-lymphocytes cultured for 5 - 10 days. The expected ED₅₀ for these effects are typically 1-5 ng/ml and 10-30 ng/ml, respectively.

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Lys22

Lyophilized

The predicted molecular weight of Recombinant Human SDF-1β is Mr 8 kDa.

8

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Muller, HW. et al. (2000) Eur J Neurosci. 12: 1857
2. Nagai, Y. et al. (1998) Proceedings of National Acad Sci USA 95: 6331