Recombinant Human TNF sRI

Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNFα are mediated.² TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses.² TNFRI is found in both membrane-bound and soluble forms. It interacts with membrane-bound or soluble forms of its ligand (TNFα), respectively. Binding of membrane-bound TNFα to the membrane-bound receptor is involved in cell survival, apoptosis, and inflammation by inducing receptor trimerization and activation. ⁴ Proteolytic processing of TNFRI yields the release of the soluble form of the receptor (TNFsRI), which inhibits inflammation via the interaction with free TNFα.

The biological activity of Soluble Tumor Necrosis Factor Receptor Type I is determined by its inhibitory effect of the TNF-Alpha mediated cytotoxicity in mouse L-929 cells. The expected ED₅₀ for this effect in the presence of 0.25 ng/ml of recombinant human TNF-Alpha is 0.05 µg/ml.

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Lyophilized

The predicted molecular weight of Recombinant Human sTNF Receptor Type I is Mr 21 kDa.

21

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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