Recombinant Human TGF-β1

Transforming growth factor beta 1 or TGF-β1 is a multifunctional cytokine that regulates the proliferation and differentiation of cells specific to bone like chondrocytes, osteoblasts, osteoclasts including mesenchymal precursor cells.¹ It is known to promote the pathogenesis of lung fibrosis and acts as a tumor suppressor in normal cells.² TGF-beta1 can promote mechanical strength in healing Achilles tendons by regulating collagen synthesis, cross-link formation, and matrix remodeling.³

The biological activity of Human Transforming Growth Factor-Beta 1 (mammalian derived) is determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED₅₀ for this effect is 0.04 - 0.2 ng/ml.

The DNA sequence encoding the human TGF-β1 precursor protein (Derynck, R. et al., 1985, Nature 316:701 - 705) was inserted into a suitable mammalian expression vector and expressed in CHO cells.

Ala279

Lyophilized

The predicted molecular weight of Recombinant Human TGF-β1 is Mr 12.8 kDa. However, the actual molecular weight as observed by migration on SDS Page is 12 kDa (reducing conditions) and 24 kDa (non-reducing conditions)

12.8

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Guven, M. et al. (2007) Growth Factors 25: 101
2. Schneider, J. et al. (2009) J Investing Med. 57(5):655-61
3. Yu, C. et al. (2009) Biochem Biophys Res Commun. 406(2):204-210