Recombinant Human TNFα
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BackgroundTNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human TNF-α was measured in a cytotoxicity assay using the TNF susceptible murine L-929 cell line in the presence of the metabolic inhibitor actinomycin D (Matthews, N. and M.L. Neale in Lymphokines and Interferons, a Practical Approach, 1987, M.J. Clemens, A.G. Morris and A.J.H. Gearing, eds., IRL Press, p. 221). The expected ED<sub>50</sub> for this effect is typically 25 - 100 pg/ml. Protein Accession No. Amino Acid Sequence vrss srtpsdkpva hvvanpqaeg qlqwlnrran allangvelr dnqlvvpseg lyliysqvlf kgqgcpsthv llthtisria vsyqtkvnll saikspcqre tpegaeakpw yepiylggvf qlekgdrlsa einrpdyldf aesgqvyfgi ial
N-terminal Sequence Analysis Met & Val77 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human TNF-α is Mr 17.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 17 kDa. Predicted Molecular Mass 17.5 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in deionized water. Storage and Stability The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Kurie, JM. et al. (2007) J Biol Chem.282(6):3507-19. Article Link 2. El-Harith, EHA. et al. (2004) Saudi Med. J. 25:135 3. Adolf, GR. et al. (1990) Infect. Immun. 58:3996 4. Larrick, JW. et al. (1990) FASEB J. 4:3215 5. Sethi, G. et al. (2009) Therapeutic Targets of the TNF Superfamily 647:37 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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