Recombinant Human TNF-β

Tumor necrosis factor-beta (TNF-β), also known as LT-α and TNFSF1, is a highly inducible, secreted protein and member of the TNF ligand superfamily. It mediates a large variety of inflammatory, immunostimulatory and antiviral responses (1). TNF-β is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis (1). It forms heteromers with lymphotoxin-beta (LT-β) that anchor the complexes to the cell surface of activated T, B and LAK cells (2). TNF-β and TNF-α are structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a wide range of similar effects (3). TNF-β promotes the proliferation of fibroblasts and induces the synthesis of GM-CSF, G-CSF, IL-1, collagenase and prostaglandin E2 (4). In neutrophils, it induces the production of reactive oxygen species, increases phagocytosis and adhesion to the endothelium, as well as acting as a chemoattractant for these cells. TNF-β is a mitogen for B-lymphocytes while it inhibits the growth of osteoclasts and keratinocytes. It is cytoxic or cytostatic for many tumor cells (6). TNF-β has been implicated in the pathogenesis of several autoimmune diseases and it plays a significant role in the etiopathogenesis of multiple sclerosis (7). Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis. TNF-β may also be involved in processes of wound healing.

The biological activity of Human Tumor Necrosis Factor-Beta is determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D. The expected ED₅₀ for this effect is 2 x 10⁷ units/mg.

mlpgvgl tpsaaqtarq hpkmhlahst lkpaahligd pskqnsllwr antdraflqd gfslsnnsll vptsgiyfvy sqvvfsgkay spkatssply lahevqlfss qypfhvplls sqkmvypglq epwlhsmyhg aafqltqgdq lsthtdgiph lvlspstvff gafal

Met

Lyophilized

The predicted molecular weight of Recombinant Human TNF-β is Mr 19 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 17-19 kDa (reducing conditions).

19

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

1. Adolf, GR. et al. (1990) Infec. Immun. 58:3996
2. Trinchieri, G. et al. (1992) Immunol. Ser. 56:289
3. Dembic, Z. et al. (1990) Cytokine 2:231
4. Oster, W. et al. (1987) Blood. 70:1700
5. Broudy, VC. et al. (1987) J. Immunol. 138:4298
6. Pagliacci, MC. et al. (1993) Lymph. Cyto. Res. 12:439
7. Pandey, JP. et al. (2002) Autoimmunity 35:377