Recombinant Human TRAIL/Apo2L

TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain,” which activates the cell's apoptotic machinery. Recombinant human soluble TRAIL/Apo2L is a 168 amino acid polypeptide (19.6 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL/Apo2L protein.

The biological activity of Human TNF-Related Apoptosis Inducing Ligand/Apo2 Ligand is determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED₅₀ for this effect is 1.0-3.0 ng/ml.

Q6IBA9

vrergpqrva ahitgtrgrs ntlsspnskn ekalgrkins wessrsghsf lsnlhlrnge lvihekgfyy iysqtyfrfq eeikentknd kqmvqyiyky tsypdpillm ksarnscwsk daeyglysiy qggifelken drifvsvtne hlidmdheas ffgaflvg

Val114 (with and without an N-terminal Met)

Lyophilized

The predicted molecular weight of Recombinant Human TRAIL/Apo2L is Mr 19 kDa.

19

Lyophilized from a 0.2 μm filtered solution in Tris, (NH₄)₂SO₄ and ZnSO₄.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

1. Rehm, M. et al. (2008) Biochim Biophys Acta. 1783(10):1903-13. Article Link