Recombinant Human TSG-6

TSG-6, also known as TNFIP6, is a secretory protein and member of the LINK-Module superfamily. It contains a hyaluronan-binding domain which is involved in extracellular matrix stability and cell migration. The expression of TSG-6 can be induced by TNF-α and IL-1 (1). It can also be induced by mechanical stimuli in vascular smooth muscle cells and is correlated with proteoglycan synthesis and aggregation (2). Cells reported to express TSG-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, endothelium and smooth muscle (2). TSG-6 forms a stable complex with inter-alpha-inhibitor (IαI) and thus enhances the serine protease inhibitory activity of IαI (3). It seems to play a critical negative regulatory feed-back function in inflammation, especially in arthritic processes (4). Growing evidence also indicates that TSG-6 acts as a crucial factor in ovulation by influencing the expansion of the hyaluronan-rich cumulus extracellular matrix in the pre-ovulatory follicle (5). TSG-6 also interacts with matrix associated molecules, such as aggregcan, versican, thrombospondin (1-2), pentraxin-3 and fibronectin.

Histidine Tag

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Trp18

Lyophilized

The predicted molecular weight of Recombinant Human TSG-6 is Mr 30.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 40 kDa.

30.5

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day 2-8°C

1. Klampfer, L. et al. (1994) Mol. Cell. Biol. 14:6561
2. Milner, CM. et al. (2003) J. Cell Sci. 116:1863
3. Forteza, R. et al. (2007) Am. J. Respir. Cell. Mol. Biol. 36:20
4. Wisniewski, HG. et al. (1996) J. Immunol. 156:1609
5. Carrette, O. et al. (2001) Biol. Reprod. 65:301