Recombinant Human TSLP

Thymic stromal lymphopoietin (TSLP) is an IL-7-like epithelial cell-derived cytokine that supports differentiation and long-term growth of B cells (1). It is expressed in the heart, liver, testis and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5 but uses kinases other than the JAKs for activation. It activates DCs primed naïve T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFα) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergic inflammation (2-3). TSLP prohibits apoptosis and stimulates growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (4).

Data not yet available.

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Met

Lyophilized

The predicted molecular weight of Recombinant Human TSLP is Mr 15 kDa.

15

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Alpers, CE. et al. (2001) Am. J. Pathol. 159:2355
2. Ziegler, SF. et al. (2007) Annu. Rev. Immunol. 25:193
3. Ziegler, SF. et al. (2005) Nat. Immunol. 6:1047
4. Gilliet, M. et al. (2003) J. Exp. Med. 197:1059