Recombinant Human VEGF-B

Vascular endothelial growth factor B (VEGF-B) is a member of the VEGF family and particularly abundant in heart and skeletal muscle.¹ It is an angiogenic and neuroprotective protein that reduces hypoxic and ischemic neuronal injury.² VEGF-B has a role in vascularization of the heart, skeletal muscles and developing bones, and in paracrine interactions between endothelial and surrounding muscle cells.³ VEGF-B also regulates lipid metabolism.⁴

The biological activity of Human VEGF-B was determined by its binding ability in a functional ELISA. Immobilized recombinant rat Neuropilin1 at 4 μg/ml (100 μl/well) can bind recombinant human VEGFB 167 with a linear range of 0.3-20 ng/ml.

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Pro22

Solution

The predicted molecular weight of Recombinant Human VEGF-B is Mr 19 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 19-22 kDa (reducing conditions).

19

This recombinant protein was 0.2 μm filtered and is supplied as a solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

The protein should be stored at -20 - -70°C upon receipt. It may be stored at 2 - 8°C for one month. However, it is recommended to aliquot into working volumes and store at -20 - -70°C in a manual defrost freezer. Avoid repeat freeze thaw cycles.

Next Day 2-8°C

1. Eriksson, U. et al. (1993) Proc Natl Acad Sci USA 93: 2576
2. Greenberg, DA. et al. (2006) Dev Biol. 289: 329
3. Eriksson, U. et al. (1999) Dev Dyn. 215: 12
4. Alitalo, K. et al. (2008) Circ Res. 103: 1018