Recombinant Human WISP-3

WNT1 inducible signaling pathway protein 3, also known as WISP3 is a secreted tumor suppressor protein.¹ It regulates the expression of cartilage-specific molecules that sustain chondrocyte growth and cartilage integrity. WISP-3 can function as a ligand and signal via autocrine and/or paracrine modes upon being secreted by chondrocytes.² WISP3 is also essential for normal postnatal skeletal growth and cartilage homeostasis.³ IT is considered a novel target in development of Microsatellite instability (MSI-H) colorectal carcinomas.⁴

The biological activity of Human WNT-1 Inducible Signaling Pathway Protein-3 is determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED₅₀ for this effect is 0.2-0.3 µg/ml.

TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQPCD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFCGICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVCQRNCRE PGDIFSELKI L

Lyophilized

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

1. Kleer CG et al. (2004) Neoplasia 6: 179 2. Sen M et al. (2001) Gastroenterology 121: 1275 3. Hurvitz JR et al. (1999) Nature Genetics 23: 94 4. Lothe RA et al. (2008) Mol Cell Biol. 28: 1114