Recombinant Influenza A NP (A/Hong Kong/1-1/1968(H3N2))
Recombinant Influenza A NP (A/Hong Kong/1-1/1968(H3N2))
Product No.: F600
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Alternate Names Influenza A Virus NP, NP, Nucleocapsid Protein, Nucleoprotein Product Type Recombinant Protein Expression Host HEK-293 Cells Species Influenza A Virus Applications ELISA ⋅ WB |
Data
Western Blot
Purified Recombinant Influenza A NP (Leinco Prod. No.: F600) was separated on SDS-PAGE under reducing conditions and probed with an anti-Influenza A NP antibody (Leinco Prod. No.: F613).
SDS-PAGE
R: 5µg reduced Recombinant Influenza A NP (Leinco Prod. No.: F600)
NR: 5µg non-reduced Recombinant Influenza A NP (Leinco Prod. No.: F600)
Direct binding of anti-Influenza A antibody (Leinco Prod. No.: F604) to Recombinant Influenza A NP (Leinco Prod. No.: F600)
Binding was measured by ELISA. Recombinant Influenza A NP was immobilized at 1 µg/mL. Anti-Influenza A NP antibody was titrated.
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BackgroundThere are four types of Influenza (flu) viruses: type A, B, C, and D. Influenza A and B viruses are routinely spread in people and other mammals, while also known for seasonal flu epidemics each year. Influenza A is the genus Alpha Influenzavirus of the virus family Orthomyxoviridae. Influenza A is classified by two main protein subtypes, hemagglutinin (HA) and neuraminidase (NA), located on the surface of the virus. Breaking down the proteins further, there are 18 hemagglutinin subtypes and 11 different neuraminidase subtypes1. Influenza A can be even further classified into specific clades (also known as groups) and sub-clades (also known as sub-groups) based on similarity of their HA/NA gene sequences1. The Nucleocapsid protein or nucleoprotein (NP) of the influenza virus A negative-strand RNA’s primary function is to encapsulate the virus genome for the purpose of RNA transcription, replication and packaging2. Specifically, NP is the most abundant viral protein in infected cells2, therefore the NP can be and has been used for anti-influenza drug development3. The NP of influenza A and B viruses share up to 38% of their amino acid sequence, indicating region functionality differences at the amino acid level4. Protein DetailsSpecies Viral Format Purified No Carrier Protein Purity ≥95% by SDS Page Product Concentration ≥ 5.0 mg/ml Endotoxin Level <1.0 EU/µg Biological Activity ELISA binding Protein Accession No. Amino Acid Sequence MASQGTKRSYEQMETDGERQNATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYKRVDGKWMRELVLYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELIRMIKRGINDRNFWRGENGRKTRSAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGPAVASGYDFEKEGYSLVGIDPFKLLQNSQVYSLIRPNENPAHKSQLVWMACNSAAFEDLRVLSFIRGTKVSPRGKLSTRGVQIASNENMDAMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISVQPAFSVQRNLPFDKPTIMAAFTGNTEGRTSDMRAEIIRMMEGAKPEEMSFQGRGVFELSDEKAANPIVPSFDMSNEGSYFFGDNAEEYDN State of Matter Frozen; PBS no preservative SDS-Page Molecular Weight 59 kDa Predicted Molecular Mass The predicted molecular mass is ~59 kDa. Predicted Molecular Mass 59 kDa Formulation This recombinant protein is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of proteins, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Storage and Stability This recombinant protein is stable for twelve months when stored at -20°C to -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Frozen Dry Ice Ligand/Receptor Ligand Species Viral Regulatory Status Research Use Only UniProt.org B3RDT3 Applications and Recommended Usage ? (Quality Tested by Leinco) SDS-PAGE, WB, ELISA References & Citations1. Types of Influenza viruses (2019) Center for Disease Control and Prevention. 2. Hu Y, Sneyd H, et al. (2017) Curr Top med Chem. 2017; 17(20): 2271–2285. 3. Portela A, Digard P. (2012) J. Gen Virol. 2002 Apr;83(Pt 4):723-734. 4. Sherry L, Smith M, et al. (2014) Journal of Virology. 2014; 88(21); 12326-12338. DOI https://doi.org/10.1128/JVI.01542-14 Technical Protocols  Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
