Recombinant Mouse CD28

CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

The biological activity of Mouse CD28 was determined by its ability to inhibit IL2 secretion by stimulated Jurkat human leukemic T cells. The expected ED₅₀ is 0.3-1.2 μg/ml when stimulated with 1 μg/ml rhB71 in the presence of PHA.

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Lys21

Lyophilized

The predicted molecular weight of Recombinant Mouse CD28 is Mr 52 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE under reducing conditions is Mr 60 kDa.

52

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Green, JM. et al. (2017) Cell Immunol. 319:28-34.