Recombinant Mouse CD30 Ligand

The CD30 ligand is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin's disease derived cell lines. The receptor for CD30L, CD30/ TNFRSF8/CD153, is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis.

Histidine Tag

mhhhhhhhhh hgggsgggsg ggsiegrqkk dstpntteka plkggncsed lfctlkstps kkswaylqvs khlnntklsw nedgtihgli yqdgnlivqf pglyfivcql qflvqcsnhs vdltlqllin skikkqtlvt vcesgvqskn iyqnlsqfll hylqvnstis vrvdnfqyvd tntfpldnvl svflysssd

Met

Lyophilized

The predicted molecular weight of Recombinant Mouse CD30L is Mr 22 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 30-45 kDa.

22

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

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2. Gruss, HJ. et al. (1994) Blood 83:1521
3. Wendtner, CM. et al. (1995) Cancer Res. 55:4157
4. Fischer, M. et al. (2006) J. Clin. Invest. 116:2748
5. Blazar, BR. et al. (2004) J. Immunol. 173:2933