Recombinant Mouse Fractalkine (FKN)

Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium¹ that mediates attachment and firm adhesion of T cells, monocytes and NK cells.² FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.³ FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).⁴

Histidine Tag

qhlgmt kceimcgkmt sripvallir yqlnqescgk raivlettqh rrfcadpkek wvqdamkhld hqaaaltkng gkfekrvdnv tpgitlatrg lspsaltkpe satledlale lttisqearg tmgtsqeppa avtgsslsts eaqdagltak pqsigsfeaa disttvwpsp avyqsgsssw aeekatesps ttapspqvst tspstpeenv gsegqppwvq gqdlspeksl gseeinpvht dnfqergpgn tvhpsvapis seetpspelv asgsqapkie epihatadpq klsvlitpvp dtqaatrhhh hhh

Gln25 predicted

Lyophilized

The predicted molecular weight of Recombinant Mouse Fractalkine is Mr 34 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 90 kDa.

34

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Patel, DD. et al. (1998) J Exp Med. 188: 1413
2. Hwang, ST. et al. (1999) Eur J Immunol. 29: 2551
3. Muller, GA. et al. (2008) Z Rheumatol. 67: 424
4. Mori, M. et al. (2005) Int J Oncol. 26: 41