Recombinant Mouse HGF R

Hepatocyte growth factor receptor (HGFR), also known as c-Met, plays an important roles in angiogenesis and tumor growth.¹ C-Met is a receptor tyrosine kinase expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC-γ. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis.^2,3

Measured by its ability to bind rmHGF in a functional ELISA type binding assay with an estimated K_D < 0.2 nM.

Fc Chimera His-tag

Mouse HGF R (Met 1 - Asn 929) - Linker peptide (DIEGRMD) - Human IgG1 (Pro 100 - Lys 330) - 6x His Tag

Glu25 ( alpha chain) & Ser307 ( beta chain)

Lyophilized

The predicted molecular weight of Recombinant Mouse HGF R is Mr 32 kDa ( alpha chain) & 96 kDa ( beta chain). However, the actual molecular weight as observed by migration on SDS-PAGE is 35-40 kDa & 100-110 kDa (reducing conditions).

Mr 32 kDa ( alpha chain) & 96 kDa ( beta chain)

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. McDonald, DM. et al. (2008) BMB Rep. 41: 833
2. Park, M. et al. (1998) Oncogene 16: 833
3. Comoglio, PM. et al. (1998) J Cell Sci. 111: 237