Recombinant Mouse ICAM-1 Fc Chimera

ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

The biological activity of Mouse ICAM-1 was determined by the ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10⁴ cells/well are added to rmICAM coated plates (12.5 μg/ml with 100 μl/well), approximately 65 - 95% will adhere after PMA stimulation for 1 hour at RT.

Fc Fusion Protein

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Gln28

Lyophilized

The predicted molecular weight of Recombinant Mouse ICAM-1 is Mr 76.8 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 110-130 kDa.

76.8

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives present.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

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4. Ozcan, U. et al. (2009) Arch Gynecol Obstet.