Recombinant Mouse ICOS

Inducible co-stimulator (ICOS), also called ALIM and H4, is a homodimeric protein and member of the CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. ICOS enhances all basic T cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. Unlike the constitutively expressed CD28, ICOS has to be de novo induced on the T cell surface, does not up-regulate the production of IL-2, but superinduces the synthesis of IL-10, a B cell-differentiation factor (1). In vivo, ICOS is highly expressed on tonsillar T cells, which are closely associated with B cells in the apical light zone of germinal centres, the site of terminal B cell maturation. ICOS regulates CD28-dependent and CD28-independent CD4 (+) subset (Th1 and Th2) responses (2). It is a major regulator of the adaptive immune system and therefore, has a protective role in inflammatory autoimmune diseases (3).

Fc Fusion Protein

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Glu21

Lyophilized

The predicted molecular weight of Recombinant Mouse ICOS is Mr 44 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 47 kDa.

44

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Flavell, RA. et al. (2001) Nature 409:97
2. Bachmann, MF. et al. (2000) J. Exp. Med. 192:53
3. Kroczek, RA. et al. (1999) Nature 397:263