Recombinant Mouse IFN-γ R1

IFN-γ receptor ligand-binding chain (IFNγR1) or IFN-γ R α chain, also known CDw119, is member of the class II cytokine receptor family. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.^1,2,3

The biological activity of Mouse IFN-γ R1 was determined by its ability to inhibit rmIFN-γ mediated protection of mouse L929 cells viral lysis. The expected ED₅₀ is typically 0.4 - 2 μg/mL in the presence of 1 ng/mL of rmIFN-γ.

Q91Y85

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Ala26

Lyophilized

The predicted molecular weight of Recombinant Mouse IFN-γ R1 is Mr 52.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 70-90 kDa.

52.4

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Calderon, J. et al. (1988) PNAS 85: 4837
2. Garcia-Sastre, A. et al. (2002) Int. Rev. Immunol. 21: 305
3. Fish, E. et al. (2002) J. Interferon Cytokine Res. 22: 835