Recombinant Mouse IGFBP-2

Insulin-like growth factor binding protein 2, also known as IGFBP2, is an extracellular unglycosylated polypeptide that specifically binds IGF and modulates its effects.¹ IGFBP-2 has direct effects on breast cancer proliferation via stimulation of critical signaling pathways.²

The biological activity of Mouse IGFBP-2 was determined by its ability to inhibit the biological activity of rmIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED₅₀ is typically 0.125 - 0.5 μg/ml in the presence of 30 ng/ml recombinant mouse IGF-II.

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Gln35

Lyophilized

The predicted molecular weight of Recombinant Mouse IGFBP-2 is Mr 29.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 35 kDa.

29.4

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Luthman, H. et al.(1991) Biochem Biophys Res Commun. 176: 1250
2. Disis, ML. et al.(2008) Cancer Res. 68: 8400