Recombinant Mouse IL-10

IL-10 is a homodimeric, anti-inflammatory cytokine of 17-21 kD with various, pleiotropic, effects in immunoregulation and inflammation. It increases antibody production, in addition to enhancing B cell survival and proliferation. IL-10 inhibits both the synthesis of pro-inflammatory cytokines and the actions of Natural Killer (NK) cells during the immune response to viral infection. Moreover, IL-10 is involved in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens. IL-10 can also block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. In mice, lack of IL-10 has been shown to cause inflammation and pain via COX activation resulting in vascular endothelial and cardiac dysfunctions. Additionally, IL-10 is linked to myokines, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis in the immune system. Exercise is known to increase circulating levels of IL-10. Hence, it is thought that physical exercise promotes an environment of anti-inflammatory cytokines. Furthermore, knockout studies of IL-10 suggest this cytokine is crucial for counteracting the hyperactive immune response in the intestinal tract. It has been reported that treatment with recombinant IL-10 producing bacteria has been beneficial in patients with Crohn's disease.

srgqysredn ncthfpvgqs hmllelrtaf sqvktffqtk dqldnilltd slmqdfkgyl gcqalsemiq fylvevmpqa ekhgpeikeh lnslgeklkt lrmrlrrchr flpcenkska veqvksdfnk lqdqgvykam nefdifinci eaymmikmks

Ser19

Solution

The predicted molecular weight of Recombinant Mouse IL-10 is Mr 18 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 17 kDa.

18

This recombinant protein solution was 0.2 µm filtered and is supplied in a sterile solution containing 10mM Tris, 75 mM NaCl.

This recombinant protein solution is stable for six months when stored desiccated at 2°C to 8°C.

Polar Packs

1. Akdis, CA. et al. (2009) Mol. Immunol. 46: 622