Recombinant Mouse IL-15

Interleukin-15 (IL-15), also known as IL-T and MGC9721, is an immunomodulating cytokine that stimulates the proliferation of T lymphocytes and shares many biological properties with IL-2 (1). IL-15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta and skeletal muscle. It exerts its biological activities primarily on T cells and is essential in the development, survival and activation of NK cells (2). IL-15 is also secreted by activated monocytes/macrophages following infection by virus (3). It competes for binding sites with IL-2, as both stimulate the growth of cells through the IL-2 receptor and thus negatively regulate each other's activity (4). IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. It can recruit and activate T lymphocytes in the synovial membrane, thereby contributing to Rheumatoid arthritis pathogenesis (5). Increased expression of IL-15 has also been implicated with inflammatory bowl disease and diseases affiliated with retroviruses HIV and HTLV-I (6). Human IL-15 is biologically active on mouse cells as measured by the dose-dependent stimulation of the proliferation of mouse CTLL cells.

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED₅₀ for this effect is typically 5 - 30 ng/mL.

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Met

Lyophilized

The predicted molecular weight of Recombinant Mouse IL-15 is Mr 13.4 kDa.

13.4

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS), Tris® mM, and NaCl 20 mM; pH 8.0 with, magnesium, or preservatives. and PBS.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.