Recombinant Mouse IL-18

Recombinant Mouse IL-18

Product No.: I-1191


Alternate Names Interleukin 18, Interferon gamma-inducing factor, IL-1 gamma Product Type Recombinant Protein Expression Host E. coli Cells Species Mouse Applications ELISA ⋅ FC ⋅ WB


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Data


Protein Details Purity >90% by SDS Page and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity Measured by its ability to induce mouse IFNg secretion by activated mouse T cells. The ED50 for this effect is 0.06-0.36 ng/mL. Protein Accession No. P70380 Amino Acid Sequence NFGRLHCTT AVIRNINDQV LFVDKRQPVF EDMTDIDQSA SEPQTRLIIY MYKDSEVRGL AVTLSVKDSK MSTLSCKNKI ISFEEMDPPE NIDDIQSDLI FFQKRVPGHN KMEFESSLYE GHFLACQKED DAFKLILKKK DENGDKSVMF TLTNLHQS N-terminal Sequence Analysis Asn36 & Leu40 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Mouse IL-18 is Mr 18.2 kDa. Predicted Molecular Mass 18 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS), with 1mM EDTA and 1 mM DTT with 5% trehalose, pH 7.2. Storage and Stability The lyophilized protein should be stored desiccated at -20 to -70°C. The reconstituted protein can be stored for at least one month at 2 to 8°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20 to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank 16173 UniProt.org Information on Mouse IL-18 on UniProt.org Applications and Recommended Usage ? (Quality Tested by Leinco) ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. Flow Cytometry:PN:R1190 Western Blotting: This antibody can be used to detect Human, Mouse and Rat TIM-1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as PN:R1190, under either reducing or non-reducing conditions. The positive control for Western blotting is PN:U124. Technical Protocols Certificate of Analysis Request COA IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.

Protein Details

Purity

>90% by SDS Page and analyzed by silver stain.

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Biological Activity

Measured by its ability to induce mouse IFNg secretion by activated mouse T cells. The ED50 for this effect is 0.06-0.36 ng/mL.

Protein Accession No.

P70380

Amino Acid Sequence

NFGRLHCTT AVIRNINDQV LFVDKRQPVF EDMTDIDQSA SEPQTRLIIY MYKDSEVRGL AVTLSVKDSK MSTLSCKNKI ISFEEMDPPE NIDDIQSDLI FFQKRVPGHN KMEFESSLYE GHFLACQKED DAFKLILKKK DENGDKSVMF TLTNLHQS

N-terminal Sequence Analysis

Asn36 & Leu40

State of Matter

Lyophilized

Predicted Molecular Mass

The predicted molecular weight of Recombinant Mouse IL-18 is Mr 18.2 kDa.

Predicted Molecular Mass

Formulation

This recombinant protein was lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS), with 1mM EDTA and 1 mM DTT with 5% trehalose, pH 7.2.

Storage and Stability

The lyophilized protein should be stored desiccated at -20 to -70°C. The reconstituted protein can be stored for at least one month at 2 to 8°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20 to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day Ambient

NCBI Gene Bank

16173

UniProt.org

Information on Mouse IL-18 on UniProt.org

Applications and Recommended Usage ?
(Quality Tested by Leinco)

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry:PN:R1190
Western Blotting: This antibody can be used to detect Human, Mouse and Rat TIM-1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as PN:R1190, under either reducing or non-reducing conditions. The positive control for Western blotting is PN:U124.