Recombinant Mouse IL-9

Interleukin 9, also known as IL9, is a TH2 cytokine¹ belonging to the group of interleukins. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. IL-9 stimulates the proliferation of a variety of hematopoietic lineages through its interaction with a receptor of the cytokine receptor superfamily.² IL-9 plays a role in the complex pathogenesis of bronchial hyperresponsiveness as a risk factor for asthma.³

Measured in a cell proliferation assay using TS1 mouse helper T cells.⁴ The ED₅₀ for this effect is 0.01‑0.03 ng/mL.

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Gln19 is predicted

Lyophilized

The predicted molecular weight of Recombinant Mouse IL-9 is Mr 14.2 kDa. However, multiple bands were observed by migration on SDS-PAGE with an actual molecular weight between 16-25 kDa (reducing conditions).

14.2

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Renauld, JC. et al. (1995) J. Immunol 154: 5061
2. Yang, YC. et al. (1995) J. Biol. Chem. 270: 20497
3. Levitt, RC. et al. (1997) Proc Natl Acad Sci USA. 94: 13175
4. Uyttenhove, C. et al. (1988) Proc. Natl. Acad. Sci. 85:6934