Recombinant Mouse/Rat MMP-2

Matrix metallopeptidase 2, also known as MMP2, is an enzyme involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-2 degrades type IV collagen, the major structural component of basement membranes and plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. MMP2 has been found to be associated with tumor invasion in various cancers.¹

The specific activity, measured under the described conditions, is > 1,500 pmoles/min/μg.

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Ile34

Solution

The predicted molecular weight of Recombinant Mouse MMP-2 is Mr 71 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 72 kDa.

71

Supplied as a 0.2 μm filtered solution of 25 mM Tris, pH 7.5, 5 mM CaCl2, 75 mM NaCl, 0.025% Brij-35 and 50% glycerol at a concentration of 0.113 mg/ml.

This protein is stable for one month when stored sterile at -20°C or for three months when stored at -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

1. Viswanadha, VP. et al. (2017) J Cell Biochem. 118(9):2865-2876.