Recombinant Mouse Myostatin

Myostatin (Mstn)is a secreted growth and differentiating factor (GDF-8) that belongs to the transforming growth factor-beta (TGF-beta) superfamily.¹ It is expressed specifically in developing and mature skeletal muscle and appears to act as a negative regulator of muscle development.² Myostatin plays a significant, direct role in bone formation and regeneration.³

The biological activity of Mouse Myostatin was determined by its ability to cause elongation of animal caps from Xenopus blastulae (Gamer, et al., 1999, Dev. Biol. 208:222). Optimal elongation was observed with 25 - 100 ng/mL recombinant mouse GDF-8.

dfg ldcdehstes rccrypltvd feafgwdwii apkrykanyc sgecefvflq kyphthlvhq anprgsagpc ctptkmspin mlyfngkeqi iygkipamvv drcgcs

Asp268

Lyophilized

The predicted molecular weight of Recombinant Mouse Myostatin is Mr 12.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 12 kDa (reducing conditions) and 24 kDa (non-reducing conditions)

12.4

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Bass, JJ. et al. (1999) J Cell Physiol. 180: 1
2. Smith, TP. et al. (2001) Anim. Genet. 29: 19
3. Hamrick, MW. et al. (2009) Bone 44: 17