Recombinant Mouse PD-L1 (B7-H1)

PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a coinhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.

The biological activity of Mouse B7-H1 was determined by its ability to inhibit anti-CD3 induced proliferation of 72 hour PHA T cell blast. The expected ED₅₀ for this effect is typically 2.5 - 10 μg/mL.

Fc Fusion Protein

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Phe19

Lyophilized

The predicted molecular weight of Recombinant Mouse B7-H1 is Mr 51.3 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 75-85 kDa.

51.3

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Sheppard, KA. et al. (2004) FEBS Letters 574:37
2. Flies, DB. et al. (2007) J. Immunol. 30:251
3. Yamazaki, T. et al. (2002) J. Immunol. 169:5538
4. Thompson, RH. et al. (2004) Proc. Natl. Acad. Sci. USA 101:17174