Recombinant Mouse PDGF-BB

Platelet-derived growth factor-BB homodimers (PDGF-BB) activates all combinations of PDGF receptor subunits, whereas PDGF-AA acts specifically on PDGFR alpha.¹ This constitutes a positive feed-back mechanism, which, for example, could serve to potentiate autocrine stimulation of growth.² PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of macrophage-colony stimulating factor (M-CSF) production in vascular smooth muscle cells.³ PDGF-BB is also a potent wound-healing hormones that accelerate incisional repair.⁴ PDGF-BB is associated with excessive cell migration, proliferation and many growth-related diseases.⁵ It may be a new prognostic indicator for patients with colorectal cancer.⁶ PDGF-BB may also induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell (CASMC) migration and proliferation through agent-specific regulation of oxidative status and utilization of different signal transduction pathways.⁵

Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED₅₀ for this effect is 1.0-2.0 ng/ml.

MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT

Lyophilized

The predicted molecular weight of the disulfide linked homodimer Recombinant Mouse PDGF-BB is Mr 24.6 kDa.

24.6

This protein was 0.2 µm filtered and lyophilized from Sodium Citrate, pH 3.0.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

1. Pfeilschifter, J. et al. (2005) Am Society of Nephrol. 10: 1681
2. Claesson-Welsh, L. et al. (1991) J Biol Chem. 266: 21138
3. Yamada, N. et al. (1992) J Biol Chem. 267: 15455
4. Mustoe, TA. et al. (1991) J Lab Clin Med. 117: 373
5. Bayraktutan, U. et al. (2008) J Mol Cell Cardiol. 45: 198
6. Mori, M. et al. (2008) Ann Surg Oncol. 15: 2129