Recombinant Mouse Sonic Hedgehog (N Terminus)

Sonic hedgehog (Shh) is a secreted factor regulating patterning of the anterior-posterior axis in the developing limb.¹ Shh functions as a secreted signaling molecule in limb pattern formation, differentiation of motor neurons, and sclerotome induction.² Shh pathway is known to be functional in the developing male tract.³ Shh may have therapeutic value as a protective agent in neurodegenerative disease.⁴

The biological activity of Mouse Sonic Hedge Hedgehog was determined by its ability to induce ALK Phos production by C3H10T1/2 mouse fibroblasts. The expected ED₅₀=0.6 - 3.0 µg/ml.

Histidine Tag

cgpgrg fgkrrhpkkl tplaykqfip nvaektlgas gryegkitrn serfkeltpn ynpdiifkde entgadrlmt qrckdklnal aisvmnqwpg vklrvtegwd edghhseesl hyegravdit tsdrdrskyg mlarlaveag fdwvyyeska hihcsvkaen svaaksgghh hhhh

Cys25

Lyophilized

The predicted molecular weight of Recombinant Mouse Shh is Mr 20 kDa.

20

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with trehalose pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Tabin, CJ. et al. (1996) Dev Biol. 180: 273
2. Noji, S. et al. (1996) Biochem Biophys Res Commun 216: 68
3. Jacobs, JP. et al. (2004) J Androl. 25: 514
4. Pang, K. et al. (1997) J Neurosci. 17: 5891