Recombinant Mouse Thrombopoietin (E. Coli Expressed)

Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.¹ TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis² and platelet production.¹

The biological activity of Mouse TPO was determined by a cell proliferation assay using MO7e cells (Avanzi, G. et al., 1988, Br. J. Haematol. 69:359). The expected ED₅₀ for this effect is typically 1 - 3 ng/ml.

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF

Lyophilized

The molecular weight of Recombinant Mouse TPO is Mr 18.7 kDa.

This recombinant protein was 0.2 µm sterile filtered and lyophilyzed in PBS pH 7.4 with no preservatives or additives.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Vainchenker W et al. (1998) Eur Cytokine Newt. 9: 221 2. Skoda RC et al. (1998) Blood 92: 4023