Recombinant Mouse Thrombopoietin (E. Coli Expressed)

Recombinant Mouse Thrombopoietin (E. Coli Expressed)

Product No.: T646

[product_table name="All Top" skus="T646"]

- -
- -
Alternate Names
THPO, MGC163194, Megakaryocyte Growth and Development Factor (MGDF), MKCSF, ML, MPLLG, MpI Ligand
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Mouse

- -
- -
Select Product Size
- -
- -

Background

Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.1 TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis2 and platelet production.1

Protein Details

Biological Activity
The biological activity of Mouse TPO was determined by a cell proliferation assay using MO7e cells (Avanzi, G. et al., 1988, Br. J. Haematol. 69:359). The expected ED<sub>50</sub> for this effect is typically 1 - 3 ng/ml.
Protein Accession No.
Amino Acid Sequence
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF
State of Matter
Lyophilized
Predicted Molecular Mass
The molecular weight of Recombinant Mouse TPO is Mr 18.7 kDa.
Formulation
This recombinant protein was 0.2 µm sterile filtered and lyophilyzed in PBS pH 7.4 with no preservatives or additives.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Vainchenker W et al. (1998) Eur Cytokine Newt. 9: 221 2. Skoda RC et al. (1998) Blood 92: 4023

Certificate of Analysis

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.