Recombinant Mouse Thrombopoietin (NS0 Expressed)

Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.¹ TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis² and platelet production.¹

The biological activity of Mouse TPO was determined by a cell proliferation assay using MO7e cells (Avanzi, G. et al., 1988, Br. J. Haematol. 69:359). The expected ED₅₀ for this effect is typically 1 - 3 ng/ml.

sspvapacdp rllnkllrds hllhsrlsqc pdvdplsipv llpavdfslg ewktqteqsk aqdilgavsl llegvmaarg qlepsclssl lgqlsgqvrl llgalqgllg tqlplqgrtt ahkdpnalfl slqqllrgkv rflllvegpt lcvrrtlptt avpsstsqll tlnkfpnrts glletnfsvt artagpglls rlqgfrvkit pgqlnqtsrs pvqisgylnr thgpvngthg lfagtslqtl easdispgaf nkgslafnlq gglppspsla pdghtpfpps palptthgsp pqlhplfpdp sttmpnstap hpvtmyphpr nlsqet

Ser22

Lyophilized

The predicted molecular weight of Recombinant Mouse TPO is Mr 35 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 80-90 kDa.

35

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Vainchenker, W. et al. (1998) Eur Cytokine Newt. 9: 221
2. Skoda, RC. et al. (1998) Blood 92: 4023