Recombinant Mouse TL1A

TL1A, also known as TNFSF15, belongs to the TNF superfamily of ligands. It is expressed predominantly in endothelial cells and to a lesser extent in placenta, lung, kidney, skeletal muscle, pancreas, small intestine and colon. Expression of TL1A is inducible by TNF and IL1-alpha but not by IFN-gamma (1). TL1A can activate NF-κB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells and may function as an angiogenesis inhibitor (2). DcR-3 and DR3 are receptors for membrane-bound and soluble forms of TL1A (1). TL1A also induces secretion of IFN-gamma and GM-CSF. Treatment of T-lymphocytes with TL1A enhances expression of IL-2 receptors and increases proliferation in response to IL-2 (3). Secretion of TL1A in lymphoid organs might contribute to RA initiation by promoting autoantibody production, and TL1A secretion stimulated by inflammatory cytokines in RA joints might be a part of a vicious circle that aggravates RA pathogenesis (4). TL1A may play an important role in a Th1-mediated disease such as Crohn's (5). Treatment of mice with TL1A strongly enhances graft-versus-host reactivity (1).

Measured in a functional ELISA by its ability to bind Recombinant Mouse DR3.

iteersepsp qqvyspprgk prahltikkq tpaphlknql salhwehdlg maftkngmky inkslvipes gdyfiysqit frgttsvcgd isrgrrpnkp dsitvvitkv adsypeparl ltgsksvcei snnwfqslyl gamfsleegd rlmvnvsdis lvdytkedkt ffgafll

Met

Lyophilized

The predicted molecular weight of Recombinant Mouse TL1A is Mr 20 kDa.

20

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.

1. Migone, TS. et al. (2002) Immunity 16:479
2. Yue, TL. et al. (1999) J. Biol. Chem. 274:1479
3. Papadakis, KA. et al. (2004) J. Immunol. 172:7002
4. Zhang, J. et al. (2009) J. Immunol. 183:5350
5. Bamias, G. et al. (2006) Proc. Natl. Acad. Sci. (USA) 103:8441