Recombinant Mouse TNFα (aa 84-235)

TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

The biological activity of Mouse TNF-α was determined in a cytotoxic assay using the TNF-susceptible murine L-929 cell line in the presence of the metabolic inhibitor actinomycin D (Matthews, N. and M.L. Neale, 1987, Lymphokines and Interferons, a practical approach, Clemens, M.J. Morris, A.G., and A.J.H. Gearing, eds., IRL Press, p. 221). The expected ED₅₀ for this effect is typically 5 - 10 pg/ml.

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Ser84

Lyophilized

The predicted molecular weight of Recombinant Mouse TNF-α is Mr 17 kDa.

17

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. El-Harith, EHA. et al. (2004) Saudi Med J. 25: 135
2. Adolf, GR. et al. (1990) Infec Immun. 58: 3996