Recombinant Rat FGF-Basic

Acidic and basic fibroblast growth factors (FGFs) are members of a family of proteins that exert pleiotropic effects in a range of cell types including skeletal myocytes.¹ Fibroblast growth factor basic (FGF basic) significantly promote angiogenesis; a central event for the growth and metastasis of solid tumors.² It is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, placenta, and monocytes, epithelial and endothelial cells. FGF-basic plays a central role during prenatal development and postnatal growth and regeneration of tissues, by promoting cellular proliferation and differentiation. FGF-basic is one of the angiogenesis factors and may promote wound healing.³

The biological activity of Rat FGF-Basic was determined by thymidine uptake by 3T3 cells expressing FGF receptors. The expected ED₅₀ is 0.1 - 10 ng/ml.

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Ala11

Lyophilized

The predicted molecular weight of Recombinant Rat FGF-Basic is Mr 16.2 kDa.

16.2

This recombinant protein was lyophilized from a 0.2 μm filtered solution in MOPS, sodium sulphate (Na₂SO₄), EDTA, Dithiothreitol (DTT).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution and addition of a carrier protein such as 0.1% BSA or HSA, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Swain, JL. et al. (1991) Developement 111: 741
2. Grevers, G. et al. (1997) Laryngorhinootologie 76: 421
3. Bühring, HJ. et al. (2007) Differentiation. 75(4):279-91