Recombinant Rat GDNF

Glial cell derived neurotrophic factor, also known as GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.¹ GDNF potently promotes the survival of many types of neurons, most prominently, dopaminergic and motorneurons. GDNF regulates kidney development and spermatogenesis, and it affects alcohol consumption.²

The biological activity of Rat GDNF was determined by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. The expected ED₅₀ is typically 1-3 ng/ml.

spdkqaaalp rrernrqaaa aspensrgkg rrgqrgknrg cvltaihlnv tdlglgyetk eelifrycsg sceaaetmyd kilknlsrsr rltsdkvgqa ccrpvafddd lsflddslvy hilrkhsakr cgci

Ser78

Lyophilized

The predicted molecular weight of Recombinant Rat GDNF is Mr 15 kDa.

15

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Collins, F. et al. (1993) Science 260: 1130
2. Ron, D. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105: 8114