Recombinant Rat IFNγ

Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.¹ It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.² IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.³

The biological activity of Rat IFN-Gamma was determined in an anti-viral assay using L-929 cells infected with EMC virus (Meager, A., 1987, Lymphokines and Interferons, a Practical Approach, M.J. Clemens, Morris, A.G. and Gearing, A.J.H., eds., IRL Press, p. 129). The expected ED₅₀ for this effect is 1 - 3 ng/ml.

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Met

Solution

The predicted molecular weight of Recombinant Rat IFN-gamma is Mr 15.5 kDa.

15.5

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

This recombinant protein solution is stable for three months when stored at 2°C to 8°C.

Polar Packs

1. Goeddel, DV. et al. (1982) Nature 298: 859
2. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
3. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163